CGA ELISA Kit

Produktnummer ABIN5652937

Dieses Human CGA ELISA-Kit ist ein Colorimetric ELISA-Kit, das dafür entwickelt wurde, Human CGA zu quantifizieren.

Kurzübersicht für CGA ELISA Kit (ABIN5652937)

Target: CGA (Glycoprotein Hormones, alpha Polypeptide (CGA))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Competition ELISA

Detektionsbereich: 123.5 pg/mL - 10000 pg/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Produktdetails CGA ELISA Kit

Untere Nachweisgrenze: 123.5 pg/mL

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Chorionic Gonadotropin Alpha Polypeptide (CGa). No significant cross-reactivity or interference between Chorionic Gonadotropin Alpha Polypeptide (CGa) and analogues was observed.

Sensitivität: 49.4 pg/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 2 h

Plattentyp: Pre-coated

Protokoll: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Chorionic Gonadotropin Alpha Polypeptide (CGa) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Chorionic Gonadotropin Alpha Polypeptide (CGa) and unlabeled Chorionic Gonadotropin Alpha Polypeptide (CGa) (Standards or samples) with the pre-coated antibody specific to Chorionic Gonadotropin Alpha Polypeptide (CGa). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Chorionic Gonadotropin Alpha Polypeptide (CGa) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Chorionic Gonadotropin Alpha Polypeptide (CGa) in the sample.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Chorionic Gonadotropin Alpha Polypeptide (CGa) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Chorionic Gonadotropin Alpha Polypeptide (CGa) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu CGA

Target: CGA (Glycoprotein Hormones, alpha Polypeptide (CGA))

Andere Bezeichnung: Chorionic Gonadotropin Alpha Polypeptide

Hintergrund:

Gene Name: Chorionic Gonadotropin Alpha Polypeptide

Gene Aliases: FSHA, GPHA1, GPHa, HCG, LHA, TSHA, Thyroid Stimulating Hormone Alpha, Follicle-Stimulating Hormone Alpha Subunit, Luteinizing hormone alpha chain, Lutropin alpha chain

Pathways: Metabolism of Steroid Hormones and Vitamin D, Thyroid Hormone Synthesis, Hormone Transport, Peptide Hormone Metabolism